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葛宏华
职 称: 教授
职 务: 课题组长(PI)
E-Mail:

ghh_ahu(AT)foxmail.com, gehonghua(AT)gmail.com

学科类别: 生物化学与分子生物学

蛋白质晶体学实验室

学习工作经历

    分别毕业于安徽大学生物化学专业(理学学士)和中国科学技术大学生化与分子生物学专业(理学博士学位)。2008-2009年赴澳大利亚Monash大学从事博士后研究工作,2013-2014年赴美国Cornell大学进行访问学者研究工作。2015年安徽省学术和技术带头人后备人选,安徽省生物工程学会理事。

主要研究领域

    1、蛋白质的结构与功能

    2、纳米生物界面传感

课题组成员

    张楠楠博士,讲师,毕业于安徽大学(学士)和中国科学技术大学(博士)

    博士研究生:陈小芳,宫晓建,Muhammad Fazal Hameed

    硕士研究生:尹士艳,刘珊,宋晓文,栗娜娜, 吴谨,唐峰,赵小雷,胡馨益,曲筱燕 

课题组承担项目

    国家自然科学基金项目(31270770,31400641)
    科技部国际科技合作专项项目(2014DFG42290)
    安徽省国际科技合作项目(1704e1002228) 

发表论文* 通讯作者)

28. Zhang N, Yin S, Zhang W, Gong X, Zhang N, Fang K, Ge H*. Crystal Structure and Biochemical Characterization of an Aminopeptidase LapB from Legionella pneumophila. J Agric Food Chem. 2017, 65 (34), pp 7569–7578.

27. Zhang N, Yin S, Liu S, Sun A, Zhou M, Gong X, Ge H*. Crystal structure of lpg1832, a VirK family protein from Legionella pneumophila reveals a novel fold for bacterial VirK proteins. FEBS LETT. 2017 Aug 3. doi: 10.1002/1873-3468.12773.

26. Wang W, Hynninen V, Qiu L, Zhang A, Lemma T, Zhang N, Ge H*, Toppari J*, Hytonen V, Wang J*. Synergistic enhancement via plasmonic nanoplate-bacteria-nanorod supercrystals for highly efficient SERS sensing of food-borne bacteria. Sensors and Actuators B: Chemical. 2017, 239:515-525

25. Qiu L, Wang W, Zhang A, Zhang N, Lemma T, Ge H*, Toppari J*, Hytonen V, Wang J*. Core-shell nanorod columnar array combined with gold nanoplate-nanosphere assemblies enable powerful in-situ SERS detection of bacteria. ACS Appl. Mater. Interfaces. 2016, 8(37):24394-24403.

24. Zhang N, Gong X, Lu M, Chen X, Qin X, Ge H*. Crystal structures of Apo and GMP bound hypoxanthine-guanine phospho-ribosyltransferase from Legionella pneumophila and the implications in gouty arthritis. J Struct Biol.2016,194(3):311-6.

23. Qiu L, Wang W, Zhang N, Jia H,  Wang J, Ge H. A versatile Anisometric metallic supercrystal with controllable orientation on a chip as stable and reliable label-free biosensor. Chem Asian J. 2016, 11(2):256-264.

22. Chen X, Hu Y, Yang B, Gong X, Zhang N, Niu L, Wu Y, Ge H*. Structure of lpg0406, a carboxymuconolactone decarboxylase family protein possibly involved in antioxidative response from Legionella pneumophila. Protein Sci. 2015, 24(12:2070-2075.

21. Jia H, Wang W, Qiu L, Zhang N, Ge H, Wang J. Fabricating a Long-Range Ordered 3D Bimetallic Nanoassembly with Edge-On Substrate for Highly Sensitive SERS Sensing of Escherichia coli Bacteria. Plasmonics. 2015, 10(6):1889-1894.

20. Zhou D, Pan Y, Chen X, Zhang N*, Ge H*. Crystallization and preliminary crystallographic analysis of the major acid phosphatase from Legionella pneumophila. Acta Crystallogr F Struct Biol Commun. 2015 Jun;71(Pt 6):779-83.

19. Wu Y, Ge H, Zhou Z. Effects of Fructus ligustri lucidi on the growth, cell integrity, and metabolic activity of the Microcystis aeruginosa. Environ Sci Pollut Res Int. 2015 Jun;22(11):8471-9.

18. Gong X, Chen X, Yu D, Zhang N, Zhu Z, Niu L, Mao Y, Ge H*. Crystal structure of Legionella pneumophila dephospho-CoA kinase reveals a non-canonical conformation of P-loop. J Struct Biol. 2014 Dec;188(3):233-9.

17. Yu D, Chen X, Xu Z*, Ge H*. Crystallization and preliminary X-ray characterization of the dephospho-CoA kinase from Legionella pneumophila. Acta Crystallogr F Struct Biol Commun. 2014 May;70(Pt 5):608-10.

16Ge H*, Chen X, Yang W, Niu L, Teng M*. Crystal structure of wild-type and mutant human Ap4A hydrolase. Biochem Biophys Res Commun. 2013 Mar 1;432(1):16-21.

15. Gao Y, Ge H, Chen H, Li H, Liu Y, Chen L, Li X, Liu J, Niu L, Teng M. Crystal structure of agkisacucetin, a Gpib-binding snake C-type lectin that inhibits platelet adhesion and aggregation. Proteins. 2012 Jun;80(6):1707-11.

14Ge H, Xu P, Xu Y, Fang Z, Xiao Y. Purification, crystallization and preliminary crystallographic analysis of recombinant Lac15 from a marine microbial metagenome. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Aug 1;67(Pt 8):956-8.

13葛宏华* , 武赟, 肖亚中. 漆酶空间结构、反应机理及应用的研究进展. 生物工程学报. 2011. 27(2):156163.

12. Li H, Tong S, Li X, Shi H, Ying Z, Gao Y, Ge H, Niu L, Teng M. Structural basis of pre-mRNA recognition by the human cleavage factor Im complex. Cell Res. 2011 Jul;21(7):1039-51.

11. Ma J, Lu Q, Yuan Y, Ge H, Li K, Zhao W, Gao Y, Niu L, Teng M. Crystal structure of isoamyl acetate-hydrolyzing esterase from Saccharomyces cerevisiaereveals a novel active site architecture and the basis of substrate specificity. Proteins. 2011 Feb;79(2):662-8.

10. Fang Z, Li T, Wang Q, Zhang X, Peng H, Fang W, Hong Y, Ge H, Xiao Y. A bacterial laccase from marine microbial metagenome exhibiting chloride tolerance and dye decolorization ability. Appl Microbiol Biotechnol. 2011 Feb;89(4):1103-10

9. Wu Y, Du X, Ge H, Lv Z. Preparation of microporous starch by glucoamylase and ultrasound. STARCH-STARKE. 2011,63:217-225.

8Ge H, Lv X, Fan J, Gao Y, Teng M, Niu L. Crystal structure of glutamate-1-semialdehyde aminotransferase from Bacillus subtiliswith bound pyridoxamine-5'-phosphate. Biochem Biophys Res Commun. 2010 Nov 12;402(2):356-60.

7Ge H, Gao Y, Hong Y, Zhang M, Xiao Y, Teng M, Niu L. Structure of native laccase B fromTrametes sp. AH28-2. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Mar 1;66(Pt 3):254-8.

6. Vivian JP, Riedmaier P, Ge H, Le Nours J, Sansom FM, Wilce MC, Byres E, Dias M, Schmidberger JW, Cowan PJ, d'Apice AJ, Hartland EL, Rossjohn J, Beddoe T. Crystal structure of a Legionella pneumophila ecto -triphosphate diphosphohydrolase, a structural and functional homolog of the eukaryotic NTPDases. Structure. 2010 Feb 10;18(2):228-38.

5. Shi ZB, Ge HH, Zhao P, Zhang M. Crystallization and preliminary crystallographic analysis of recombinant VSP1 from Arabidopsis thaliana. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Feb 1;66(Pt 2):201-3.

4. Zhou D, Ge H, Sun J, Gao Y, Teng M, Niu L. Crystal structure of the C-terminal conserved domain of human GRP, a galectin- related protein, reveals a function mode different from those of galectins. Proteins. 2008 May 15;71(3):1582-8.

3Ge H, Zhou D, Tong S, Gao Y, Teng M, Niu L. Crystal structure and possible dimerization of the single RRM of human PABPN1. Proteins. 2008 May 15;71(3):1539-45.

2. Lv X, Fan J, Ge H, Gao Y, Zhang X, Teng M, Niu L. Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of the glutamate-1-semialdehyde aminotransferase from Bacillus subtilis. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 May 1;62(Pt 5):483-5.

1. Li X, Guo M, Fan J, Tang W, Wang D, Ge H, Rong H, Teng M, Niu L, Liu Q, Hao Q. Crystal structure of 3-hydroxyanthranilic acid 3,4-dioxygenase from Saccharomyces cerevisiae: a special subgroup of the type III extradiol dioxygenases. Protein Sci. 2006 Apr;15(4):761-73.  

 

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